Staining:Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.
Article Snippet: .. Fig. 1 GLP-1RA improves glucose homeostasis in HFD mice and insulin secretion in PA-treated NIT-1 cells (a) Body weight, (b) fasting glucose, (c) food intake (n = 5) (d) IPGTT and AUC of IPGTT (n = 5) (e) IPITT and AUC of IPITT (n = 5) (f) IPIRT (n = 5) (g) Glucose stimulation index (fold change of basal insulin secretion in the IPIRT) (n = 5) (h) Pancreatic islet insulin staining (IF: INSULINgreen, DAPI-blue; Magnification 400×, scale bar = 50 μm) (i) GSIS upon basal (2.8 mmol/L) or high (16.7 mmol/L) glucose conditions of NIT-1 cells (n = 3) (j) Insulin-secretion gene expression (quantitative PCR) in NIT-1 cells (n = 3) (k, l) Insulin-secretion proteins (Western blot) and quantification in NIT-1 cells (n = 4) Panel a-h: vs. NC + saline (*P < 0.05, **P < 0.01); vs. HFD + saline (†P < 0.05, ††P < 0.01); vs. basal serum insulin concentration (‡P < 0.05, ‡‡P < 0.01); Panel i-l: vs. Ctrl (*P < 0.05, **P < 0.01); vs. PA (†P < 0.05, ††P < 0.01) 1 3 375 Page 2 of 20 GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity... 1 3 Page 3 of 20 375 Z. Liu et al. Gibco) supplemented with 15% (v/v) foetal bovine serum (10099141 C, Gibco). ..
Gene Expression:Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.
Article Snippet: .. Fig. 1 GLP-1RA improves glucose homeostasis in HFD mice and insulin secretion in PA-treated NIT-1 cells (a) Body weight, (b) fasting glucose, (c) food intake (n = 5) (d) IPGTT and AUC of IPGTT (n = 5) (e) IPITT and AUC of IPITT (n = 5) (f) IPIRT (n = 5) (g) Glucose stimulation index (fold change of basal insulin secretion in the IPIRT) (n = 5) (h) Pancreatic islet insulin staining (IF: INSULINgreen, DAPI-blue; Magnification 400×, scale bar = 50 μm) (i) GSIS upon basal (2.8 mmol/L) or high (16.7 mmol/L) glucose conditions of NIT-1 cells (n = 3) (j) Insulin-secretion gene expression (quantitative PCR) in NIT-1 cells (n = 3) (k, l) Insulin-secretion proteins (Western blot) and quantification in NIT-1 cells (n = 4) Panel a-h: vs. NC + saline (*P < 0.05, **P < 0.01); vs. HFD + saline (†P < 0.05, ††P < 0.01); vs. basal serum insulin concentration (‡P < 0.05, ‡‡P < 0.01); Panel i-l: vs. Ctrl (*P < 0.05, **P < 0.01); vs. PA (†P < 0.05, ††P < 0.01) 1 3 375 Page 2 of 20 GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity... 1 3 Page 3 of 20 375 Z. Liu et al. Gibco) supplemented with 15% (v/v) foetal bovine serum (10099141 C, Gibco). ..
Real-time Polymerase Chain Reaction:Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.
Article Snippet: .. Fig. 1 GLP-1RA improves glucose homeostasis in HFD mice and insulin secretion in PA-treated NIT-1 cells (a) Body weight, (b) fasting glucose, (c) food intake (n = 5) (d) IPGTT and AUC of IPGTT (n = 5) (e) IPITT and AUC of IPITT (n = 5) (f) IPIRT (n = 5) (g) Glucose stimulation index (fold change of basal insulin secretion in the IPIRT) (n = 5) (h) Pancreatic islet insulin staining (IF: INSULINgreen, DAPI-blue; Magnification 400×, scale bar = 50 μm) (i) GSIS upon basal (2.8 mmol/L) or high (16.7 mmol/L) glucose conditions of NIT-1 cells (n = 3) (j) Insulin-secretion gene expression (quantitative PCR) in NIT-1 cells (n = 3) (k, l) Insulin-secretion proteins (Western blot) and quantification in NIT-1 cells (n = 4) Panel a-h: vs. NC + saline (*P < 0.05, **P < 0.01); vs. HFD + saline (†P < 0.05, ††P < 0.01); vs. basal serum insulin concentration (‡P < 0.05, ‡‡P < 0.01); Panel i-l: vs. Ctrl (*P < 0.05, **P < 0.01); vs. PA (†P < 0.05, ††P < 0.01) 1 3 375 Page 2 of 20 GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity... 1 3 Page 3 of 20 375 Z. Liu et al. Gibco) supplemented with 15% (v/v) foetal bovine serum (10099141 C, Gibco). ..
Western Blot:Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.
Article Snippet: .. Fig. 1 GLP-1RA improves glucose homeostasis in HFD mice and insulin secretion in PA-treated NIT-1 cells (a) Body weight, (b) fasting glucose, (c) food intake (n = 5) (d) IPGTT and AUC of IPGTT (n = 5) (e) IPITT and AUC of IPITT (n = 5) (f) IPIRT (n = 5) (g) Glucose stimulation index (fold change of basal insulin secretion in the IPIRT) (n = 5) (h) Pancreatic islet insulin staining (IF: INSULINgreen, DAPI-blue; Magnification 400×, scale bar = 50 μm) (i) GSIS upon basal (2.8 mmol/L) or high (16.7 mmol/L) glucose conditions of NIT-1 cells (n = 3) (j) Insulin-secretion gene expression (quantitative PCR) in NIT-1 cells (n = 3) (k, l) Insulin-secretion proteins (Western blot) and quantification in NIT-1 cells (n = 4) Panel a-h: vs. NC + saline (*P < 0.05, **P < 0.01); vs. HFD + saline (†P < 0.05, ††P < 0.01); vs. basal serum insulin concentration (‡P < 0.05, ‡‡P < 0.01); Panel i-l: vs. Ctrl (*P < 0.05, **P < 0.01); vs. PA (†P < 0.05, ††P < 0.01) 1 3 375 Page 2 of 20 GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity... 1 3 Page 3 of 20 375 Z. Liu et al. Gibco) supplemented with 15% (v/v) foetal bovine serum (10099141 C, Gibco). ..
Saline:Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.
Article Snippet: .. Fig. 1 GLP-1RA improves glucose homeostasis in HFD mice and insulin secretion in PA-treated NIT-1 cells (a) Body weight, (b) fasting glucose, (c) food intake (n = 5) (d) IPGTT and AUC of IPGTT (n = 5) (e) IPITT and AUC of IPITT (n = 5) (f) IPIRT (n = 5) (g) Glucose stimulation index (fold change of basal insulin secretion in the IPIRT) (n = 5) (h) Pancreatic islet insulin staining (IF: INSULINgreen, DAPI-blue; Magnification 400×, scale bar = 50 μm) (i) GSIS upon basal (2.8 mmol/L) or high (16.7 mmol/L) glucose conditions of NIT-1 cells (n = 3) (j) Insulin-secretion gene expression (quantitative PCR) in NIT-1 cells (n = 3) (k, l) Insulin-secretion proteins (Western blot) and quantification in NIT-1 cells (n = 4) Panel a-h: vs. NC + saline (*P < 0.05, **P < 0.01); vs. HFD + saline (†P < 0.05, ††P < 0.01); vs. basal serum insulin concentration (‡P < 0.05, ‡‡P < 0.01); Panel i-l: vs. Ctrl (*P < 0.05, **P < 0.01); vs. PA (†P < 0.05, ††P < 0.01) 1 3 375 Page 2 of 20 GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity... 1 3 Page 3 of 20 375 Z. Liu et al. Gibco) supplemented with 15% (v/v) foetal bovine serum (10099141 C, Gibco). ..
Concentration Assay:Article Title: GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity via PPARδ/UCP2 pathway.
Article Snippet: .. Fig. 1 GLP-1RA improves glucose homeostasis in HFD mice and insulin secretion in PA-treated NIT-1 cells (a) Body weight, (b) fasting glucose, (c) food intake (n = 5) (d) IPGTT and AUC of IPGTT (n = 5) (e) IPITT and AUC of IPITT (n = 5) (f) IPIRT (n = 5) (g) Glucose stimulation index (fold change of basal insulin secretion in the IPIRT) (n = 5) (h) Pancreatic islet insulin staining (IF: INSULINgreen, DAPI-blue; Magnification 400×, scale bar = 50 μm) (i) GSIS upon basal (2.8 mmol/L) or high (16.7 mmol/L) glucose conditions of NIT-1 cells (n = 3) (j) Insulin-secretion gene expression (quantitative PCR) in NIT-1 cells (n = 3) (k, l) Insulin-secretion proteins (Western blot) and quantification in NIT-1 cells (n = 4) Panel a-h: vs. NC + saline (*P < 0.05, **P < 0.01); vs. HFD + saline (†P < 0.05, ††P < 0.01); vs. basal serum insulin concentration (‡P < 0.05, ‡‡P < 0.01); Panel i-l: vs. Ctrl (*P < 0.05, **P < 0.01); vs. PA (†P < 0.05, ††P < 0.01) 1 3 375 Page 2 of 20 GLP-1 receptor agonist protects glucose-stimulated insulin secretion in pancreatic β-cells against lipotoxicity... 1 3 Page 3 of 20 375 Z. Liu et al. Gibco) supplemented with 15% (v/v) foetal bovine serum (10099141 C, Gibco). ..
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 